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Protein ALignment Optimiser (PALO) is an algorithm for the selection of the best combination of protein isoforms among orthologous genes in the construction of a multiple alignment. You can easily upload your files from ENSEMBL and this tool will tell you which is the most suitable combination for you to align.
Take a look to the HOW-TO section if this is your first visit. You can either use this online version (section Run) or download the raw code and run it in your local machine